- Lillian Gish
- Elsie Stoneman
- Mae Marsh
- Flora Cameron
- Henry B. Walthall
- Col. Ben Cameron
- Miriam Cooper
- Margaret Cameron
- Mary Alden
- Lydia Brown
- Ralph Lewis
- Austin Stoneman
- George Siegmann
- Silas Lynch
- Walter Long
- Gus
- Robert Harron
- Tod Stoneman
- Wallace Reid
- Jeff (blacksmith)
- Joseph Henabery
- Abraham Lincoln/13 other bits, Assistant Director, Film Editing
- Elmer Clifton
- Phil Stoneman
- Josephine Crowell
- Mrs. Cameron
- Spottiswoode Aitken
- Dr. Cameron
- George Beranger
- Wade Cameron
- Maxfield Stanley
- Duke Cameron
- Jennie Lee
- Mammy
- Donald Crisp
- Gen. Ulysses S. Grant
- Howard Gaye
- Gen. Robert E. Lee
- Sam De Grasse
- Sen. Sumner (uncredited)
- Raoul Walsh
- John Wilkes Booth (uncredited), Assistant Director, Film Editing
- Eugene Pallette
- Union soldier (uncredited)
- Elmo Lincoln
- Blacksmith (uncredited)
- Olga Grey
- Laura Keene (uncredited)
- William De Vaull
- Nelse (uncredited)
- Tom Wilson
- Stoneman's servant (uncredited)
- Violet Wilkey
- Flora as a child (uncredited)
- Alberta Lee
- Mrs. Lincoln (uncredited)
- D.W. Griffith
- Director, Film Editing, Original Music, Producer, Writer
- Thomas F. Dixon Jr.
- Writer
- Frank E. Woods
- Writer
- G.W. Bitzer
- Cinematographer
- Joseph Henabery
- Abraham Lincoln/13 other bits, Assistant Director, Film Editing
- James Smith
- Film Editing
- Rose Smith
- Film Editing
- Raoul Walsh
- John Wilkes Booth (uncredited), Assistant Director, Film Editing
- Joseph Carl Breil
- Original Music
- Edvard Grieg
- Non-Original Music
- Richard Wagner
- Non-Original Music
